Let us denote the integral by
$$I_n=\int_0^1\frac{x^{2n-3}}{(1+x^2)^n}\mathrm{d}x.$$
Before we move on let us make the observations that
$$I_{n+1}=\int_0^1\frac{x^{2n-1}}{(1+x^2)^{n+1}}\mathrm{d}x$$
and
$$\frac{\mathrm{d}}{\mathrm{d}x}\left(\frac{1}{(1+x^2)^n}\right)=-\frac{2xn}{(1+x^2)^{n+1}}.$$
This gives us a small hint that maybe integration by parts will help us out somehow, so let us give it a try. Integration by parts (where we differentiate what I already differentiated and integrate what is left) gives us that
\begin{align*}
I_n
&= \int_0^1\frac{x^{2n-3}}{(1+x^2)^n}\mathrm{d}x \\
&= \left[\frac{x^{2n-2}}{2n-2}\cdot\frac{1}{(1+x^2)^n}\right]_0^1+\int_0^1\frac{x^{2n-2}}{2n-2}\cdot\frac{2xn}{(1+x^2)^{n+1}}\mathrm{d}x \\
&= \frac{1}{2^{n+1}(n-1)}+\frac{n}{n-1}\int_0^1\frac{x^{2n-1}}{(1+x^2)^{n+1}}\mathrm{d}x.
\end{align*}
Now notice that something great happened: integration by parts gave us back something familiar! Indeed what we have now is that
$$I_n=\frac{1}{2^{n+1}(n-1)}+\frac{n}{n-1}I_{n+1},$$
which we can rearrange to get
$$I_{n+1}=\frac{n-1}{n}I_n-\frac{1}{2^{n+1}n}.$$
There is a subtilty in getting here to keep in mind though. If $n=1$, then the antiderivative of $x^{2n-3}$ is not $x^{2n-2}$, so this would only give us the solutions when $n\geq 2$ (you can probably spot that $n=1$ also gives problems in the original recursive formula we found). To finish this part off notice that, using the substitution you tried before, we can find that
$$I_2=\int_0^1\frac{x}{(1+x^2)^2}\mathrm{d}x=\frac{1}{4}.$$
Thus we have that the integral is recursively given by
$$\begin{cases}\displaystyle
I_{n+1}=\frac{n-1}{n}I_n-\frac{1}{2^{n+1}n}, & n\geq 2, \\
I_2 = \frac{1}{4}
\end{cases}.$$
This of course does not give you a closed form, but hopefully it gives you some valuable insight into the problem and how you can tackle problems like this, and if you really want a closed form, try to solve the recursion formula and see if it gets you anywhere.