I've been thinking about Monsky's Theorem that it is impossible to partition a square into an odd number of triangles of equal area. The proof depends on a theorem of Chevalley to extend the $2$-adic valaution on $\mathbb{Q}$ to a $2$-adic valuation on $\mathbb{R}$, and Chevalley's theorem relies on the axiom of choice for its proof. According to "Proofs from THE BOOK" (fifth edition, p.$151$) no other proof of Monsky's theorem is known.
If Monsky's theorem were false, the counterexample would be a finite, easily comprehensible object. All the other applications of the axiom of choice I can think of assert the existence of some infinite, complicated object that we don't expect to be able to construct: a Hamel basis for the reals over the rationals, a free ultrafilter, a bounded, non-measurable subset of the reals, and so on.
I recognize that Chevalley's theorem itself asserts the existence of just such an object, but I haven't been able to think of another example where the axiom or one of its consequences is used to prove the non-existence of some "concrete" finite object. I exclude the extensions of Monsky's theorem to other polygons and higher dimensions, of course.
Are such examples really rare? Can you supply any more?