Let $E_1:=\begin{bmatrix}1&0\\0&0\end{bmatrix}$, $E_1:=\begin{bmatrix}0&1\\0&0\end{bmatrix}$, $E_3:=\begin{bmatrix}0&0\\1&0\end{bmatrix}$, and $E_4:=\begin{bmatrix}0&0\\0&1\end{bmatrix}$. Then, the matrix of transformation sending $(E_1,E_2,E_3,E_4)$ to $(A_1,A_2,A_3,A_4)$ is
$$T:=\begin{bmatrix}1&1&1&1\\0&1&1&1\\0&0&1&1\\0&0&0&1\end{bmatrix}\,.$$
The required bilinear form in the basis $(A_1,A_2,A_3,A_4)$ is given by the matrix
$$B:=\begin{bmatrix}1&0&0&0\\0&4&0&0\\0&0&9&0\\0&0&0&16\end{bmatrix}\,.$$
Therefore, in the basis $(E_1,E_2,E_3,E_4)$, the bilinear form is given by the matrix
$$\left(T^{-1}\right)^\top\,B\,T^{-1}=\begin{bmatrix}1&-1&0&0\\-1&5&-4&0\\0&-4&13&-9\\0&0&-9&25\end{bmatrix}\,.$$
In other words, this bilinear form is given by
$$\left\langle \begin{bmatrix}a&b\\c&d\end{bmatrix},\begin{bmatrix}x&y\\z&w\end{bmatrix}\right\rangle=ax-ay-bx+5by-4bz-4cy+13cz-9cw-9dz+25dw$$
for $a,b,c,d,x,y,z,w\in\mathbb{R}$.
If the base field is $\mathbb{C}$, then the bilinear form (or rather, the sesquilinear form) is given by
$$\left\langle \begin{bmatrix}a&b\\c&d\end{bmatrix},\begin{bmatrix}x&y\\z&w\end{bmatrix}\right\rangle=a\bar x-a\bar y-b\bar x+5b\bar y-4b\bar z-4c\bar y+13c\bar z-9c\bar w-9d\bar z+25d\bar w$$
for $a,b,c,d,x,y,z,w\in\mathbb{C}$.