Say that I have two uncertain parameters, A and B. I estimate each population's mean and variance using estimators of the mean, $Q_A:=\frac{1}{N_A}\sum_{i=1}^{N_A}a_i, a_i \sim A$ and $Q_B:=\frac{1}{N_B}\sum_{i=1}^{N_B}b_i, b_i \sim B$, and variance, $\hat{s}_A:=\sum_{i=1}^{N_A}\frac{(a_i-Q_A)^2}{N_A}$ and $\hat{s}_B:=\sum_{i=1}^{N_B}\frac{(b_i-Q_B)^2}{N_B}$.
Say that I want to estimate $\frac{{\mathrm{Var}}(A)}{\mathrm{Var}(B)}$. If I'm doing Monte Carlo simulation and $N_a$>$N_b$, then I can either approximate it using
$\frac{{\mathrm{Var}}(A)}{\mathrm{Var}(B)} \approx \frac{\hat{s}_A}{\hat{s}_B}$,
or I can approximate it using the identical samples,
$\frac{{\mathrm{Var}}(A)}{\mathrm{Var}(B)} \approx \sum_{i=1}^{N_B} \frac{(a_i-Q_A)^2}{(b_i-Q_B)^2}$.
Is there any advantages or disadvantages to one of these approaches to the other? Is one expected to be more accurate?