It is enough to prove that there is an $m>0$ such that
$$
J(u)\ge m \quad \forall\ u \in H_0^1(0,1),
$$
where the functional $J$ is defined by
$$
J: H_0^1(0,1) \to [0,\infty),\ J(u)=\|u'\|_{L^2(0,1)}^2.
$$
For every $u \in H_0^1(0,1)$ we have
\begin{eqnarray}
\int_0^1u^2(x)\,dx&=&\int_0^1\Big(\int_0^xu'(t)\,dt\Big)^2\,dx\le\int_0^1\Big(\int_0^x|u'(t)|\,dt\Big)^2\,dx\\
&\le&\int_0^1x\int_0^x(u'(t))^2\,dt\,dx\le\int_0^1x\int_0^1(u'(t))^2\,dt\,dx=\frac12\int_0^1(u'(x))^2\,dx.
\end{eqnarray}
It follows that
$$
J(u) \ge 2 \quad \forall\ u \in H_0^1(0,1), \|u\|_{L^2(0,1)}=1,
$$
i.e. $J$ is bounded below on $M=\{u \in H_0^1(0,1):\ \|u\|_{L^2(0,1)}^2=1\}$.
So, let
$$
m=\inf_MJ.
$$
If there is a $u \in M$ such that $J(u)=m$, then by the Lagrange multipliers rule there exists some scalar $\lambda$ such that
$$
\int_0^1u'\phi'=\lambda\int_0^1u\phi \quad \forall\ \phi \in C_0^1(0,1),
$$
i.e. $\lambda$ and $u$ solve the problem
$$\tag{1}
u''+\lambda u=0 \ \text{ in } (0,1), \ u(0)=0=u(1).
$$
Solving (1) we get
$$
u(x)=u_\lambda(x)=a\sin(\sqrt{\lambda}x),\ \lambda \in \{k^2\pi^2:\ k \in \mathbb{N}\},\ a\in \mathbb{R}.
$$
Hence $m=\pi^2$, i.e.
$$
\int_0^1u^2(x)\,dx\le \frac{1}{\pi^2}\int_0^1(u')^2(x)\,dx \quad \forall u \in H_0^1(0,1).
$$
Another approach is to use Fourier analysis. Since $\phi_k(x)=\sqrt{2}\sin(k\pi x), k \in \mathbb{N}$ form a basis for $H_0^1(0,1)$, any $u \in H_0^1(0,1)$ can be written as
$$
u=\sum_{k=1}^\infty a_k(u)\phi_k,\ a_k(u)=\int_0^1u(x)\phi_k(x)\,dx.
$$
Hence, for every $u \in H_0^1(0,1)$ we have
$$
\int_0^1(u')^2(x)\,dx=\sum_{k=1}^\infty k^2\pi^2a_k^2(u)\ge \pi^2\sum_{k=1}^\infty a_k^2(u)=\pi^2\int_0^1u^2(x)\,dx,
$$
i.e.
$$
\int_0^1u^2(x)\,dx\le \frac{1}{\pi^2}\int_0^1(u')^2(x)\,dx \quad \forall\ u \in H_0^1(0,1).
$$
Remark The constant $m=\frac{1}{\pi^2}$ is optimal, and
$$
\int_0^1u^2(x)\,dx=\frac{1}{\pi^2}\int_0^1(u')^2(x)\,dx \iff u(x)=a\sin(\pi x), \ a \in \mathbb{R}.
$$