If $M$ is a smooth manifold and $TM$ is the tangent bundle clearly $T_pM\cong T_qM$ (as vector spaces) for every $p,q\in M$. Nobody ensures that the previous vector spaces isomorphism is natural (or canonical). In $\mathbb R^n$ we have that $T_p\mathbb R^n$ and $T_q\mathbb R^n$ are naturally isomorphic to $\mathbb R^n$ so we can differentiate a vector field along a direction, in the usual way so taking the directional derivatives of each component.
If the isomorphism between tangent spaces in different point isn't natural, why can't we differentiate a vector field in the usual way? The problem is comparing vectors belonging in different (isomorphic) vector spaces; but we can send the two vectors, with an isomorphism, in a common vector space and then subtract them. Where is the importance of a natural isomorphism?